7WBZ

Crystal structure of the SARS-Cov-2 RBD in complex with Fab 2303

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	85.134, 149.983, 144.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.090 - 2.420
R-factor	0.1841
Rwork	0.182
R-free	0.22010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7kfv
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.090	20.000	2.440
High resolution limit [Å]	2.400	6.440	2.400
Rmerge	0.190	0.099	0.751
Rmeas	0.199	0.103	0.789
Rpim	0.057	0.030	0.236
Number of reflections	35465	1892	1745
<I/σ(I)>	2.6
Completeness [%]	99.9	100	98.9
Redundancy	12.1	12.1	10.2
CC(1/2)		0.992	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		309	0.2 M sodium tartrate dibasic dihydrate, 14% (w/v) polyethylene glycol 3350