7WBZ
Crystal structure of the SARS-Cov-2 RBD in complex with Fab 2303
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 85.134, 149.983, 144.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.090 - 2.420 |
R-factor | 0.1841 |
Rwork | 0.182 |
R-free | 0.22010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7kfv |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.090 | 20.000 | 2.440 |
High resolution limit [Å] | 2.400 | 6.440 | 2.400 |
Rmerge | 0.190 | 0.099 | 0.751 |
Rmeas | 0.199 | 0.103 | 0.789 |
Rpim | 0.057 | 0.030 | 0.236 |
Number of reflections | 35465 | 1892 | 1745 |
<I/σ(I)> | 2.6 | ||
Completeness [%] | 99.9 | 100 | 98.9 |
Redundancy | 12.1 | 12.1 | 10.2 |
CC(1/2) | 0.992 | 0.819 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 309 | 0.2 M sodium tartrate dibasic dihydrate, 14% (w/v) polyethylene glycol 3350 |