7W5C
Crystal structure of Mitogen Activated Protein Kinase 4 (MPK4) from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-27 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.987 |
Spacegroup name | I 2 3 |
Unit cell lengths | 132.950, 132.950, 132.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.602 - 2.201 |
R-factor | 0.1786 |
Rwork | 0.176 |
R-free | 0.22540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2erk |
RMSD bond length | 0.010 |
RMSD bond angle | 1.091 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.602 | 2.280 |
High resolution limit [Å] | 2.201 | 2.201 |
Number of reflections | 19910 | 1920 |
<I/σ(I)> | 42.91 | |
Completeness [%] | 99.7 | |
Redundancy | 23.6 | |
CC(1/2) | 0.779 | 0.473 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2% v/v TacsimateTM pH 7.0, 0.1 M HEPES pH 7.5, 20% w/v Polyethylene glycol 3350 |