7W3O

Crystal structure of human CYB5R3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	95
Detector technology	PIXEL
Collection date	2020-02-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	163.360, 79.920, 169.400
Unit cell angles	90.00, 98.72, 90.00

Refinement procedure

Resolution	49.569 - 2.460
R-factor	0.2327
Rwork	0.231
R-free	0.25950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w5h
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.569	2.610
High resolution limit [Å]	2.460	2.460
Rmerge	0.259	1.780
Number of reflections	78626	2536
<I/σ(I)>	12.5
Completeness [%]	100.0
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% PEG 1500, 10% 2-propanol, 0.1M bicine at pH 8.5