7VVH

Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease mutation E140G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193.15
Detector technology	PIXEL
Collection date	2018-09-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 2 21
Unit cell lengths	40.775, 63.882, 79.417
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.273 - 2.296
R-factor	0.2421
Rwork	0.239
R-free	0.27240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v0c
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Refinement software	PHENIX (1.14_3247)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.296	6.240	2.300
Rmerge	0.075	0.056	0.794
Rmeas	0.082	0.063	0.881
Rpim	0.034	0.027	0.374
Number of reflections	9688	559	478
<I/σ(I)>	5.8
Completeness [%]	99.9	99.3	99.4
Redundancy	6.1	5.4	5.3
CC(1/2)		0.994	0.846

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	291	25% PEG 1500, 0.1M MMT pH 9.0