7VOI

Structure of the human CNOT1(MIF4G)-CNOT6L-CNOT7 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.977
Spacegroup name	P 41 21 2
Unit cell lengths	110.989, 110.989, 242.904
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.740 - 4.380
R-factor	0.2687
Rwork	0.263
R-free	0.32390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4b8c 4gmj
RMSD bond length	0.006
RMSD bond angle	0.961
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.480
High resolution limit [Å]	4.380	4.380
Rpim	0.050	0.177
Number of reflections	10302	493
<I/σ(I)>	27.3
Completeness [%]	100.0
Redundancy	12.3
CC(1/2)	0.990	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.1 M MES pH 6.0, 8% PEG 20000