7VN9

Crystal structure of human coronavirus 229E spike protein receptor-binding domain in complex with C04 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-05-11
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9789
Spacegroup name	P 62 2 2
Unit cell lengths	183.732, 183.732, 255.988
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.660 - 4.490
R-factor	0.2742
Rwork	0.273
R-free	0.28850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6atk 6df2
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.660
High resolution limit [Å]	4.490	4.490
Rmeas		1.802
Rpim		0.544
Number of reflections	15864	1514
<I/σ(I)>	6.75	1
Completeness [%]	100.0	100
Redundancy	16.6	10.4
CC(1/2)	0.992	0.527

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	1.6M AmSO4, 0.1M Critic Acid pH 5.0