7VKQ

Crystal structure of D. melanogaster SAMTOR in the SAH bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	49.934, 63.992, 80.268
Unit cell angles	90.26, 93.38, 96.79

Refinement procedure

Resolution	41.022 - 2.094
R-factor	0.1676
Rwork	0.166
R-free	0.20620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vkk
RMSD bond length	0.007
RMSD bond angle	0.940
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.490	2.140
High resolution limit [Å]	2.090	2.090
Rmerge	0.105	0.540
Number of reflections	54989	4909
<I/σ(I)>	6.1	1.7
Completeness [%]	94.8	78.5
Redundancy	2
CC(1/2)	0.993	0.678

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5	290	0.1 M sodium citrate, pH 5.0, and 20% (w/v) PEG 8,000.