7VJV

Human AlkB homolog ALKBH6 in complex with alpha-katoglutarate and Mn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-10
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	46.118, 64.407, 88.416
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.417 - 1.750
Rwork	0.190
R-free	0.20680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3thp
RMSD bond length	0.009
RMSD bond angle	1.495
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.780
High resolution limit [Å]	1.750	1.750
Number of reflections	27487	2473
<I/σ(I)>	15
Completeness [%]	98.7
Redundancy	4.5
CC(1/2)	0.977	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	PEG 8000, Tris