7VHY

Crystal structure of EP300 HAT domain in complex with compound (+)-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-04-10
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.541800
Spacegroup name	P 1
Unit cell lengths	44.490, 88.781, 89.949
Unit cell angles	116.58, 99.44, 85.30

Refinement procedure

Resolution	19.900 - 2.300
R-factor	0.2141
Rwork	0.212
R-free	0.25020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bhw
Data reduction software	CrystalClear
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.900	19.900	2.440
High resolution limit [Å]	2.300	6.830	2.300
Rmerge	0.112	0.023	1.013
Rmeas	0.151	0.030	1.369
Total number of observations	111641
Number of reflections	50945	1943	7613
<I/σ(I)>	7.55	31.56	0.84
Completeness [%]	93.8	93.5	86.8
Redundancy	2.191	2.182	2.105
CC(1/2)	0.991	0.999	0.371

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	15% PEG3350, 0.1 M HEPES (pH7.0)