7VGE
Structure of the PDZ deleted variant of HtrA2 protease (S306A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2020-12-31 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 82.880, 82.880, 395.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.530 - 4.000 |
R-factor | 0.26859 |
Rwork | 0.265 |
R-free | 0.33642 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lcy |
RMSD bond length | 0.006 |
RMSD bond angle | 1.497 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 4.100 |
High resolution limit [Å] | 4.000 | 4.000 |
Number of reflections | 11300 | 1441 |
<I/σ(I)> | 4.36 | 1.27 |
Completeness [%] | 90.7 | 90.9 |
Redundancy | 4.24 | 3.78 |
CC(1/2) | 0.967 | 0.251 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | 0.5 M Sodium acetate trihydrate pH 6.0, 2.0 M Sodium formate, 3% glycerol |