7VEC
Crystal structure of GABARAP complexed with the TEX264 LIR phosphorylated at Ser271 and Ser272
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-04 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54178 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 200.480, 201.383, 211.816 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.890 - 3.000 |
| R-factor | 0.2594 |
| Rwork | 0.257 |
| R-free | 0.30070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yir |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.671 |
| Data reduction software | CrysalisPro (1.171.40.14a) |
| Data scaling software | Aimless |
| Phasing software | MrBUMP |
| Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.480 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 42776 | 4477 |
| <I/σ(I)> | 6.5 | 1.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.7 | 8 |
| CC(1/2) | 0.964 | 0.608 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG 4000, 0.2M ammonium acetate, 0.1M bis-Tris pH 5.6 |
