7VBT
Crystal structure of RIOK2 in complex with CQ211
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-12-20 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9897 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.517, 93.605, 110.965 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.803 - 2.540 |
R-factor | 0.224129822459 |
Rwork | 0.221 |
R-free | 0.25916 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6hk6 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.850 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692+SVN) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.540 | 2.540 |
Number of reflections | 26038 | 2539 |
<I/σ(I)> | 19.8 | |
Completeness [%] | 100.0 | |
Redundancy | 8.3 | |
CC(1/2) | 0.953 | 0.920 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION, RECRYSTALLIZATION | 293 | 50 mM MES (pH 5.5), 3% isopropanol, 10 mM MgCl2 |