7V92
Crystal Structure of a thermostable mutant of the Catalytic Domain of GH19 Chitinase from Gazyumaru, Ficus microcarpa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2017-11-27 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.579, 38.894, 177.739 |
| Unit cell angles | 90.00, 96.03, 90.00 |
Refinement procedure
| Resolution | 47.580 - 1.607 |
| Rwork | 0.214 |
| R-free | 0.24370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tx7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.356 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.580 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 799385 | 235922 |
| <I/σ(I)> | 12 | |
| Completeness [%] | 96.6 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.998 | 0.922 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 18% (w/v) Polyethylene glycol 20K, 0.1 M sodium citrate (pH 5.0), and 3% (v/v) Glycerol. |
