7V91
Crystal Structure of the Catalytic Domain of a Family GH19 Chitinase from Gazyumaru, Ficus microcarpa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.900, 106.583, 107.656 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.094 - 1.600 |
Rwork | 0.227 |
R-free | 0.25280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tx7 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.061 | 0.381 |
Number of reflections | 1000279 | 137756 |
<I/σ(I)> | 46 | |
Completeness [%] | 99.8 | |
Redundancy | 7.3 | |
CC(1/2) | 0.937 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 3% (w/v) gamma-polyglutamic acid low molecule, 25%(w/v) 2-Methy-2, 4-pentanediol, 0.1 M HEPES (pH 7.5), 0.5 M Ammonium sulfate |