7V8F

Crystal structure of UBE2L3 bound to HOIP RING1 domain.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9789
Spacegroup name	P 1 21 1
Unit cell lengths	30.097, 108.412, 37.156
Unit cell angles	90.00, 106.33, 90.00

Refinement procedure

Resolution	54.210 - 1.660
R-factor	0.1763
Rwork	0.175
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4q5h
RMSD bond length	0.009
RMSD bond angle	1.070
Data reduction software	XDS (20190806)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	108.410	1.690
High resolution limit [Å]	1.660	1.660
Rpim	0.014	0.130
Number of reflections	24757	1078
<I/σ(I)>	30.4
Completeness [%]	91.8	80
Redundancy	6.3
CC(1/2)	0.999	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8	289	2%(v/v) 1,4-dioxane, 0.1 M Tris-HCl (pH 8.0), 15%(w/v) PEG 3,350.