7V6F
Structure of Candida albicans Fructose-1,6-bisphosphate aldolase complexed with G3P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.458, 56.570, 131.747 |
| Unit cell angles | 90.00, 113.43, 90.00 |
Refinement procedure
| Resolution | 43.680 - 2.980 |
| R-factor | 0.2204 |
| Rwork | 0.217 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lnk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.490 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.680 | 2.988 |
| High resolution limit [Å] | 2.885 | 2.885 |
| Number of reflections | 18393 | |
| <I/σ(I)> | 22.7 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.999 | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM Hepes pH =7.5, 11% (w/v) PEG 3350 |
