7V5L
Crystal structure of human bleomycin hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-08-10 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 |
| Unit cell lengths | 181.944, 181.944, 166.307 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.470 - 1.740 |
| R-factor | 0.1671 |
| Rwork | 0.165 |
| R-free | 0.20020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cb5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.471 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.800 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 209843 | 21057 |
| <I/σ(I)> | 22.9 | 2.7 |
| Completeness [%] | 99.7 | |
| Redundancy | 4.7 | |
| CC(1/2) | 0.990 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M sodium chloride, 10% (w/v) PEG 8000, 0.1mM CHES, pH 9.5 |
