7V3D
Complex structure of serine hydroxymethyltransferase from Enterococcus faecium and its inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-13 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 119.470, 119.470, 163.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.210 - 2.280 |
| R-factor | 0.1763 |
| Rwork | 0.175 |
| R-free | 0.20860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wxb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.058 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.210 | 2.420 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmeas | 0.450 | |
| Number of reflections | 54560 | 8764 |
| <I/σ(I)> | 8.12 | |
| Completeness [%] | 100.0 | |
| Redundancy | 16.8 | |
| CC(1/2) | 0.570 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | Na cacodylate trihydrate, Ammonium sulfate, Calcium chloride |
