7V37
Crystal structure of apo-NP exonuclease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97622 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 74.129, 121.276, 137.722 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.739 - 2.403 |
R-factor | 0.2109 |
Rwork | 0.208 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4g9z |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.490 |
High resolution limit [Å] | 2.400 | 5.160 | 2.400 |
Rmerge | 0.101 | 0.046 | 0.494 |
Rmeas | 0.113 | 0.052 | 0.574 |
Rpim | 0.050 | 0.022 | 0.285 |
Total number of observations | 111142 | ||
Number of reflections | 24007 | 2487 | 2333 |
<I/σ(I)> | 7.5 | ||
Completeness [%] | 98.8 | 96.9 | 98.3 |
Redundancy | 4.6 | 5.1 | 3.7 |
CC(1/2) | 0.998 | 0.723 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 38% v/v Polyethylene glycol 200, 10 mM Aurintricarboxylic acid |