7UYN
Crystal structure of B-form alien DNA 5'-CTTBPPBBSSZZSAAG in a host-guest complex with the N-terminal fragment of Moloney murine leukemia virus reverse transcriptase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 54.854, 146.480, 46.656 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.460 - 1.650 |
| R-factor | 0.2233 |
| Rwork | 0.222 |
| R-free | 0.24640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mik |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 73.240 | 73.240 | 1.680 |
| High resolution limit [Å] | 1.650 | 9.040 | 1.650 |
| Rmerge | 0.099 | 0.058 | 1.339 |
| Rmeas | 0.104 | 0.060 | 1.402 |
| Rpim | 0.029 | 0.017 | 0.408 |
| Total number of observations | 578693 | 3594 | 26107 |
| Number of reflections | 46199 | 349 | 2255 |
| <I/σ(I)> | 14.7 | 29.5 | 2.3 |
| Completeness [%] | 99.9 | 99.9 | 99.9 |
| Redundancy | 12.5 | 10.3 | 11.6 |
| CC(1/2) | 0.998 | 0.997 | 0.697 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.05 M ADA, pH 6.5, 9% PEG4000 |
