6MIK

Crystal structure of host-guest complex with PP hachimoji DNA

Summary for 6MIK

• Entry DOI: 10.2210/pdb6mik/pdb
• Descriptor: N-terminal fragment of MMLV reverse transcriptase, DNA (5'-D(*CP*TP*TP*AP*TP*(1WA)P*(1WA)P*(DS))-3'), DNA (5'-D(P*(DB)P*(1W5)P*(1W5)P*AP*TP*AP*AP*G)-3'), ... (4 entities in total)
• Functional Keywords: host-guest complex, hachimoji dna, n-terminal fragment of mmlv rt, dna, hydrolase-dna complex, hydrolase/dna
• Biological source: Moloney murine leukemia virus (isolate Shinnick) (MoMLV); More
• Total number of polymer chains: 3
• Total formula weight: 34307.07

Authors

Georgiadis, M.M. (deposition date: 2018-09-19, release date: 2019-02-27, Last modification date: 2024-03-13)

Primary citation

Hoshika, S.,Leal, N.A.,Kim, M.J.,Kim, M.S.,Karalkar, N.B.,Kim, H.J.,Bates, A.M.,Watkins Jr., N.E.,SantaLucia, H.A.,Meyer, A.J.,DasGupta, S.,Piccirilli, J.A.,Ellington, A.D.,SantaLucia Jr., J.,Georgiadis, M.M.,Benner, S.A.
Hachimoji DNA and RNA: A genetic system with eight building blocks.
Science, 363:884-887, 2019

PubMed Abstract: We report DNA- and RNA-like systems built from eight nucleotide "letters" (hence the name "hachimoji") that form four orthogonal pairs. These synthetic systems meet the structural requirements needed to support Darwinian evolution, including a polyelectrolyte backbone, predictable thermodynamic stability, and stereoregular building blocks that fit a Schrödinger aperiodic crystal. Measured thermodynamic parameters predict the stability of hachimoji duplexes, allowing hachimoji DNA to increase the information density of natural terran DNA. Three crystal structures show that the synthetic building blocks do not perturb the aperiodic crystal seen in the DNA double helix. Hachimoji DNA was then transcribed to give hachimoji RNA in the form of a functioning fluorescent hachimoji aptamer. These results expand the scope of molecular structures that might support life, including life throughout the cosmos.

PubMed: 30792304
DOI: 10.1126/science.aat0971

Experimental method

X-RAY DIFFRACTION (1.7 Å)