7UXP

Structure of PDL1 in complex with FP28132, a Helicon Polypeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-02
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97629
Spacegroup name	I 2 2 2
Unit cell lengths	83.310, 85.889, 86.859
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.800 - 2.620
R-factor	0.2457
Rwork	0.244
R-free	0.28580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zqk
RMSD bond length	0.010
RMSD bond angle	1.144
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.800	2.710
High resolution limit [Å]	2.620	2.620
Number of reflections	9672	953
<I/σ(I)>	11.7
Completeness [%]	99.9
Redundancy	10
CC(1/2)	0.996	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1 M MES pH 6.5, 1.6 M Magnesium sulfate.