7UW0
Crystal structure of human ClpP protease in complex with TR-133
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-08-03 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.98 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 105.660, 153.798, 133.208 |
Unit cell angles | 90.00, 107.03, 90.00 |
Refinement procedure
Resolution | 51.690 - 2.800 |
R-factor | 0.2623 |
Rwork | 0.260 |
R-free | 0.30880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bba |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 93.620 | 2.890 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 47509 | 4706 |
<I/σ(I)> | 4.2 | |
Completeness [%] | 95.2 | |
Redundancy | 4.9 | |
CC(1/2) | 0.805 | 0.420 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 sodium acetate, pH 4.6 to 5.2, 5% PEG 4000 |