7UUJ
Crystal structure of aminoglycoside resistance enzyme ApmA, complex with gentamicin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97943 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.763, 107.128, 137.079 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.850 - 1.780 |
| R-factor | 0.1727 |
| Rwork | 0.172 |
| R-free | 0.22120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7jm2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.109 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.074 | 0.807 |
| Rpim | 0.032 | 0.376 |
| Number of reflections | 83620 | 3882 |
| <I/σ(I)> | 19.09 | 1 |
| Completeness [%] | 96.2 | 91 |
| Redundancy | 6.1 | |
| CC(1/2) | 0.999 | 0.878 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.2 M calcium chloride dihydrate, 16% PEG3350, 2.5 mM gentamicin |
