7UUJ

Crystal structure of aminoglycoside resistance enzyme ApmA, complex with gentamicin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97943
Spacegroup name	P 21 21 21
Unit cell lengths	60.763, 107.128, 137.079
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.850 - 1.780
R-factor	0.1727
Rwork	0.172
R-free	0.22120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jm2
RMSD bond length	0.011
RMSD bond angle	1.109
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.810
High resolution limit [Å]	1.780	1.780
Rmerge	0.074	0.807
Rpim	0.032	0.376
Number of reflections	83620	3882
<I/σ(I)>	19.09	1
Completeness [%]	96.2	91
Redundancy	6.1
CC(1/2)	0.999	0.878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.2 M calcium chloride dihydrate, 16% PEG3350, 2.5 mM gentamicin