7UT3

Crystal structure of complex of Fab, G10C with GalNAc-pNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 61 2 2
Unit cell lengths	132.594, 132.594, 169.054
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.540 - 3.000
R-factor	0.1884
Rwork	0.186
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6z7x 1sy6
RMSD bond length	0.006
RMSD bond angle	1.344
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.050
High resolution limit [Å]	3.000	3.000
Number of reflections	18216	875
<I/σ(I)>	23.1
Completeness [%]	99.8	99.1
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.5M Ammonium Phosphate