7UT0
Human DDAH-1, apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.598, 80.938, 73.883 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 40.040 - 1.680 |
| R-factor | 0.1539 |
| Rwork | 0.151 |
| R-free | 0.20790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7usz |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.900 | 1.710 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmeas | 0.066 | 0.584 |
| Rpim | 0.046 | 0.397 |
| Number of reflections | 62098 | 3164 |
| <I/σ(I)> | 12.6 | 2.1 |
| Completeness [%] | 97.6 | 99.2 |
| Redundancy | 3.3 | |
| CC(1/2) | 0.977 | 0.857 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 100 mM Tris-HCl (pH 8.5), 300 mM sodium acetate, 21% PEG6000 |
