7USG
BRD2-BD2 in complex with MDP5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-07-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9787 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 52.560, 72.277, 32.213 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.600 - 1.200 |
R-factor | 0.1783 |
Rwork | 0.177 |
R-free | 0.19730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6e6j |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.600 | 1.243 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.106 | |
Rpim | 0.039 | |
Number of reflections | 35996 | 3501 |
<I/σ(I)> | 28.7 | |
Completeness [%] | 91.8 | |
Redundancy | 6.7 | |
CC(1/2) | 0.991 | 0.762 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298.15 | 0.1 M Bis-Tris pH 6.5, 16 % W/V PEG 3350 |