7URG
cryo-EM structure of ribonucleotide reductase from Synechococcus phage S-CBP4 bound with TTP
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS TALOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.46 |
| Number of Particle | 581884 |
| Software | cryoSPARC,PHENIX,SerialEM |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
