7UQ6

tRNA T-box antiterminator fusion, construct #4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	88.576, 165.208, 57.047
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.213 - 2.951
R-factor	0.2475
Rwork	0.247
R-free	0.26480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mgm
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.213	32.213	3.110
High resolution limit [Å]	2.951	9.330	2.951
Rmerge		0.053	0.552
Rmeas	0.139	0.075	0.662
Rpim	0.055	0.032	0.277
Total number of observations	56484
Number of reflections	9103	312	1263
<I/σ(I)>	18.9	9.4	1.2
Completeness [%]	99.5	95.5	97.5
Redundancy	6.2	5.5	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	50 mM sodium cacodylate pH 6.0, 80 mM KCl, 12 mM spermine, 20 mM BaCl2, 15% 2-methyl-2,4-pentanediol