7UPM

Tribbles (TRIB2) pseudokinase bound to nanobody Nb4.103

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 65 2 2
Unit cell lengths	75.160, 75.160, 273.770
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.670 - 2.700
R-factor	0.20676
Rwork	0.202
R-free	0.25967
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cem
RMSD bond length	0.004
RMSD bond angle	0.955
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.670	2.770
High resolution limit [Å]	2.700	2.700
Rmerge	0.167
Number of reflections	13514	135
<I/σ(I)>	10.2
Completeness [%]	99.7
Redundancy	6.3
CC(1/2)	0.997	0.502

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	291	0.2 M Magnesium nitrate hexahydrate, 20% w/v Polyethylene glycol 3350