7UPM
Tribbles (TRIB2) pseudokinase bound to nanobody Nb4.103
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 75.160, 75.160, 273.770 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.670 - 2.700 |
R-factor | 0.20676 |
Rwork | 0.202 |
R-free | 0.25967 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cem |
RMSD bond length | 0.004 |
RMSD bond angle | 0.955 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.670 | 2.770 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.167 | |
Number of reflections | 13514 | 135 |
<I/σ(I)> | 10.2 | |
Completeness [%] | 99.7 | |
Redundancy | 6.3 | |
CC(1/2) | 0.997 | 0.502 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 291 | 0.2 M Magnesium nitrate hexahydrate, 20% w/v Polyethylene glycol 3350 |