7UOS
Structure of WNK1 inhibitor complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 163 |
| Detector technology | CCD |
| Collection date | 2020-07-18 |
| Detector | APEX II CCD |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.820, 57.532, 65.631 |
| Unit cell angles | 90.00, 90.58, 90.00 |
Refinement procedure
| Resolution | 37.850 - 2.900 |
| R-factor | 0.22942 |
| Rwork | 0.222 |
| R-free | 0.29464 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5drb |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.777 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.850 | 2.900 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rpim | 0.060 | |
| Number of reflections | 4894 | 4894 |
| <I/σ(I)> | 10.4 | |
| Completeness [%] | 86.0 | |
| Redundancy | 4.6 | |
| CC(1/2) | 0.950 | 0.950 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 289 | 20% PEG3350, 200mM Sodium Formate |
