7UN5
Structure of Type II Prion filaments from Gerstmann-Straussler-Scheinker disease
EM specimen
| Vitrification | Yes |
| Instrument | GATAN CRYOPLUNGE 3 |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.13 |
| Software | RELION,PHENIX,Rosetta,Leginon |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
