7ULI
Apo HMG-CoA Reductase from Arabidopsis thaliana (HMG1)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 85.578, 85.578, 266.654 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.302 - 1.900 |
| Rwork | 0.207 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hw8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.357 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.302 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 39592 | 2488 |
| <I/σ(I)> | 14.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.3 | |
| CC(1/2) | 1.000 | 0.549 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M ammonium sulfate, 0.1 M HEPES, 35% w/v poly(acrylic acid sodium salt)2100 |
