Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-06-19 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 32 |
| Unit cell lengths | 58.234, 58.234, 275.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.449 |
| Rwork | 0.206 |
| R-free | 0.22822 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ufx |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.82) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.480 |
| High resolution limit [Å] | 1.449 | 1.449 |
| Rpim | 0.045 | |
| Number of reflections | 144124 | 9171 |
| <I/σ(I)> | 21 | |
| Completeness [%] | 96.4 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.570 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | PEG 8,000, Tris pH 6.5, MgCl2 |
