7UJ1

Crystal structure of PSF-RNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 3 2 1
Unit cell lengths	186.738, 186.738, 55.372
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.670 - 3.500
R-factor	0.1687
Rwork	0.165
R-free	0.24130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wii
RMSD bond length	0.007
RMSD bond angle	1.952
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.670	47.670	3.590
High resolution limit [Å]	3.500	15.650	3.500
Rmerge	0.142	0.034	2.692
Rmeas	0.161	0.039	3.092
Total number of observations	121547
Number of reflections	27221	301	2005
<I/σ(I)>	7.5	35.82	0.56
Completeness [%]	99.9	96.8	100
Redundancy	4.465	4.518	4.19
CC(1/2)	0.997	0.998	0.126

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	0.1M Tris-HCl, pH 8.5, 5mM MgCl2, 1mM DTT, 26% SOKALAN CP 42.