7UIS
Cocrystal structure of human CaMKII-alpha (CAMK2A)kinase domain and GluN2B(S1303D)
Replaces: 7KL4Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-09 |
Detector | DECTRIS PILATUS3 R 200K-A |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.413, 71.426, 45.276 |
Unit cell angles | 90.00, 97.51, 90.00 |
Refinement procedure
Resolution | 24.370 - 2.580 |
R-factor | 0.2198 |
Rwork | 0.217 |
R-free | 0.26750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6vzk |
RMSD bond length | 0.003 |
RMSD bond angle | 1.269 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
High resolution limit [Å] | 2.580 | 7.050 | 2.600 |
Rmerge | 0.162 | 0.086 | 0.678 |
Rmeas | 0.194 | 0.107 | 0.804 |
Rpim | 0.105 | 0.062 | 0.429 |
Number of reflections | 8402 | 437 | 405 |
<I/σ(I)> | 5.8 | ||
Completeness [%] | 96.9 | 95.8 | 92.5 |
Redundancy | 3.3 | 2.9 | 3.4 |
CC(1/2) | 0.979 | 0.743 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1 M Bis-tris propane pH 6.5, 0.2 M Sodium chloride, 25% PEG 3350 |