7UDG

Integrin alpha IIB beta3 complex with lotrafiban

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.996
Spacegroup name	P 21 21 2
Unit cell lengths	256.760, 144.170, 104.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.144 - 2.800
R-factor	0.213321122371
Rwork	0.213
R-free	0.23494
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3t3p
RMSD bond length	0.006
RMSD bond angle	0.605
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.870
High resolution limit [Å]	2.800	2.800
Number of reflections	183986	13682
<I/σ(I)>	4.66
Completeness [%]	99.6
Redundancy	5
CC(1/2)	0.983	0.141

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.9	277	11% PEG 8000, 0.2 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.9