7UCW

Structure of mouse Decr1 in complex with 2'-5' oligoadenylate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-03-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	82.379, 117.191, 232.248
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.390 - 1.350
R-factor	0.1512
Rwork	0.151
R-free	0.15980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w6u
RMSD bond length	0.008
RMSD bond angle	1.084
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.390	1.370
High resolution limit [Å]	1.350	1.350
Rpim	0.031	0.559
Number of reflections	244100	11679
<I/σ(I)>	10.6	1.2
Completeness [%]	99.8
Redundancy	6.8
CC(1/2)	0.998	0.525

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	34% PEG-300, 100 mM Tris-HCl pH 8.6