7UBR

Integrin alpha IIB beta3 complex with GR144053

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 2
Unit cell lengths	257.890, 144.400, 104.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.257 - 2.050
R-factor	0.20877448628
Rwork	0.209
R-free	0.22258
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3t3p
RMSD bond length	0.007
RMSD bond angle	1.104
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.050	2.050
Number of reflections	455646	30954
<I/σ(I)>	4.72
Completeness [%]	96.0	88.1
Redundancy	1.8
CC(1/2)	0.993	0.118

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.9	277	11% PEG 8000, 0.2 M ammonium sulfate, 0.1 M Tris-HCl