7UAJ

Crystal structure of apo HPV16 E6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2020-11-05
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00
Spacegroup name	P 1
Unit cell lengths	84.110, 88.680, 109.610
Unit cell angles	106.61, 90.16, 102.76

Refinement procedure

Resolution	52.390 - 3.250
R-factor	0.2572
Rwork	0.256
R-free	0.28240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xr8
Data reduction software	MOSFLM (v 7.3.0)
Data scaling software	Aimless (v 0.7.4)
Phasing software	PHASER (v 2.8.3)
Refinement software	PHENIX (v 1.20.1-4887-000)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	82.670	82.670	3.370
High resolution limit [Å]	3.250	12.160	3.250
Rmerge	0.123	0.054	1.689
Rmeas	0.144	0.067	1.960
Rpim	0.075	0.040	0.994
Total number of observations	159101	3169	17073
Number of reflections	42715	857	4578
<I/σ(I)>	5.7	13.6	0.9
Completeness [%]	91.9	97.2	93.3
Redundancy	3.7	3.7	3.7
CC(1/2)	0.919	0.886	0.401

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M HEPES, 8% ethylene glycol and 10% PEG 8000 at pH 7.5