7UAF

Human meprin alpha inhibitory complex with compound 10d (N~3~,N~3~-bis[(2H-1,3-benzodioxol-5-yl)methyl]-N-hydroxy-beta-alaninamide)

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Energy Filter	GIF Bioquantum
Detector	GATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.4
Number of Particle	925458
Software	crYOLO,Coot,ISOLDE,PHENIX,RELION,cryoSPARC,EPU,CTFFIND,Warp,UCSF ChimeraX
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION