7U79

Human DNA polymerase eta-DNA ternary mismatch complex:reaction with 1.0 mM Mg2+ for 140s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-11-28
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9787
Spacegroup name	P 61
Unit cell lengths	98.687, 98.687, 82.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.730 - 1.690
R-factor	0.1778
Rwork	0.176
R-free	0.22060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ecq
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.730	42.730	1.800
High resolution limit [Å]	1.690	5.050	1.690
Rmerge	0.074	0.022	1.801
Rmeas	0.081	0.024	1.983
Total number of observations	570099
Number of reflections	98869	3772	15727
<I/σ(I)>	14.17	58.79	0.93
Completeness [%]	99.1	99.9	97.5
Redundancy	5.766	5.643	5.671
CC(1/2)	0.999	1.000	0.344

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	10% PEG 2000MME, 0.1 M MES