7U62
Crystal structure of Anti-Heroin Antibody HY4-1F9 Fab Complexed with Morphine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-10-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.792, 97.179, 76.132 |
Unit cell angles | 90.00, 91.23, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.840 |
High resolution limit [Å] | 1.810 | 4.910 | 1.810 |
Rmerge | 0.061 | 0.061 | 0.276 |
Rmeas | 0.072 | 0.072 | 0.332 |
Rpim | 0.038 | 0.038 | 0.182 |
Total number of observations | 262956 | ||
Number of reflections | 76214 | 3916 | 3689 |
<I/σ(I)> | 13.3 | ||
Completeness [%] | 99.0 | 99.2 | 97 |
Redundancy | 3.5 | 3.6 | 3.1 |
CC(1/2) | 0.986 | 0.902 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20% PEG 8000 0.1M Sodium Cacodylate 0.2M Magnesium Acetate 2.25% Xylitol |