7U4W
The ubiquitin-associated domain of human thirty-eight negative kinase-1 flexibly fused to the 1TEL crystallization chaperone via a 2-glycine linker and crystallized at traditional protein concentration
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 65 |
Unit cell lengths | 67.971, 67.971, 55.749 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.480 - 2.100 |
R-factor | 0.2469 |
Rwork | 0.245 |
R-free | 0.27560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2QAR (for 1TEL) homology model (UBA) |
RMSD bond length | 0.004 |
RMSD bond angle | 0.565 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.480 | 2.175 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.041 | 1.088 |
Rmeas | 0.042 | 1.129 |
Rpim | 0.011 | 0.297 |
Number of reflections | 8522 | 820 |
<I/σ(I)> | 28.14 | 2.27 |
Completeness [%] | 98.3 | 96.24 |
Redundancy | 14.5 | 14.1 |
CC(1/2) | 1.000 | 0.923 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 1.2 uL of 15 mg/mL protein combined with 1.2 uL of 100 mM Bis-Tris, pH 6.50, 750 mM Mg-Formate |