7U4E
Neuraminidase from influenza virus A/Bilthoven/17938/1969(H3N2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-11-20 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9787 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 116.339, 137.944, 138.246 |
Unit cell angles | 90.00, 92.15, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.540 |
R-factor | 0.1665 |
Rwork | 0.166 |
R-free | 0.18030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2aep |
RMSD bond length | 0.003 |
RMSD bond angle | 0.995 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.049 | 1.563 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.104 | 0.989 |
Rmeas | 0.111 | 1.064 |
Number of reflections | 322550 | 322494 |
<I/σ(I)> | 12.1 | 2.1 |
Completeness [%] | 100.0 | |
Redundancy | 7.5 | |
CC(1/2) | 0.998 | 0.778 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 10% PEG-6000, 5% MPD, 0.1 M HEPES pH 6.5 |