7U44

[F344] Self-assembling tensegrity triangle with three turns, four turns and four turns of DNA per axis by extension with P1 symmetry

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-14
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00743
Spacegroup name	P 1
Unit cell lengths	100.417, 135.336, 135.190
Unit cell angles	99.31, 95.66, 106.50

Refinement procedure

Resolution	38.270 - 8.460
R-factor	0.1615
Rwork	0.160
R-free	0.19230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gbi
RMSD bond length	0.013
RMSD bond angle	1.324
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	95.149	95.149	9.719
High resolution limit [Å]	8.460	24.828	8.460
Number of reflections	3477	205	205
<I/σ(I)>	11.8
Completeness [%]	82.6
Redundancy	3.5	3.2	3.7
CC(1/2)	1.000	1.000	0.046

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	100 mM MOPS, 125 mM magnesium sulfate, sodium hydroxide