7U42
[F334] Self-assembling tensegrity triangle with three turns, three turns and four turns of DNA per axis by extension with P1 symmetry
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-14 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 1.00743 |
Spacegroup name | P 1 |
Unit cell lengths | 99.719, 101.835, 135.725 |
Unit cell angles | 99.19, 106.43, 101.55 |
Refinement procedure
Resolution | 39.010 - 7.710 |
R-factor | 0.0977 |
Rwork | 0.097 |
R-free | 0.10790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gbi |
RMSD bond length | 0.017 |
RMSD bond angle | 1.393 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 97.096 | 97.096 | 8.470 |
High resolution limit [Å] | 7.710 | 22.640 | 7.710 |
Number of reflections | 3664 | 203 | 204 |
<I/σ(I)> | 9.5 | ||
Completeness [%] | 85.3 | ||
Redundancy | 3.6 | 3.3 | 3.8 |
CC(1/2) | 0.999 | 0.999 | 0.502 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 100 mM MOPS, 125 mM magnesium sulfate, sodium hydroxide |