7U3W
[L224] Self-assembling tensegrity triangle with two turns, two turns and four turns of DNA per axis by linker addition with P1 symmetry
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-14 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.00743 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.475, 68.558, 131.418 |
| Unit cell angles | 77.89, 79.65, 83.53 |
Refinement procedure
| Resolution | 36.490 - 6.330 |
| R-factor | 0.1385 |
| Rwork | 0.137 |
| R-free | 0.15160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gbi |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.542 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 66.830 | 66.830 | 7.191 |
| High resolution limit [Å] | 6.330 | 17.662 | 6.335 |
| Rpim | 1.129 | ||
| Number of reflections | 3023 | 202 | 202 |
| <I/σ(I)> | 8.3 | ||
| Completeness [%] | 80.9 | ||
| Redundancy | 3.6 | 3.7 | 3.6 |
| CC(1/2) | 0.999 | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | MOPS, Magnesium sulfate, Sodium hydroxide |
