7U3U

[L223] Self-assembling tensegrity triangle with two turns, two turns and three turns of DNA per axis by linker addition with P1 symmetry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-18
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.00744
Spacegroup name	P 1
Unit cell lengths	67.156, 69.165, 99.464
Unit cell angles	107.84, 96.45, 97.03

Refinement procedure

Resolution	45.600 - 4.460
R-factor	0.2249
Rwork	0.224
R-free	0.24020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gbi
RMSD bond length	0.006
RMSD bond angle	0.913
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.807	65.807	5.039
High resolution limit [Å]	4.460	14.374	4.464
Number of reflections	5741	286	287
<I/σ(I)>	3.7
Completeness [%]	84.5
Redundancy	3.7	3.7	3.4
CC(1/2)	0.995	0.994	0.464

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	MOPS, Magnesium sulfate, Sodium hydroxide