7U3T

[F223] Self-assembling tensegrity triangle with two turns, two turns and three turns of DNA per axis by extension with P1 symmetry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-21
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00743
Spacegroup name	P 1
Unit cell lengths	67.406, 68.539, 100.038
Unit cell angles	98.99, 99.62, 98.58

Refinement procedure

Resolution	37.020 - 4.690
R-factor	0.2173
Rwork	0.214
R-free	0.27620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gbi
RMSD bond length	0.006
RMSD bond angle	0.846
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	66.590	66.590	4.990
High resolution limit [Å]	4.546	13.892	4.546
Number of reflections	6698	335	336
<I/σ(I)>	13.7
Completeness [%]	84.9
Redundancy	3.6		3.6
CC(1/2)	0.999	0.999	0.089

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	MOPS, Magnesium sulfate, Sodium hydroxide