7U3T
[F223] Self-assembling tensegrity triangle with two turns, two turns and three turns of DNA per axis by extension with P1 symmetry
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-21 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.00743 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.406, 68.539, 100.038 |
| Unit cell angles | 98.99, 99.62, 98.58 |
Refinement procedure
| Resolution | 37.020 - 4.690 |
| R-factor | 0.2173 |
| Rwork | 0.214 |
| R-free | 0.27620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gbi |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.846 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 66.590 | 66.590 | 4.990 |
| High resolution limit [Å] | 4.546 | 13.892 | 4.546 |
| Number of reflections | 6698 | 335 | 336 |
| <I/σ(I)> | 13.7 | ||
| Completeness [%] | 84.9 | ||
| Redundancy | 3.6 | 3.6 | |
| CC(1/2) | 0.999 | 0.999 | 0.089 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | MOPS, Magnesium sulfate, Sodium hydroxide |
